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Addgene

pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR
(Plasmid #207805)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207805 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    #73022
  • Backbone manufacturer
    addgene
  • Backbone size w/o insert (bp) 8689
  • Total vector size (bp) 10933
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E-NoMi
  • Alt name
    EF-1alpha driven EV reporter called Nanoluc outside mCherry inside
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2133
  • Promoter EF-1alpha
  • Tags / Fusion Proteins
    • nanoluciferase
    • 3xFlag tag
    • mCherry (C terminal on insert)
    • copGFP (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATGGCGGTGGAAGGAGGAA
  • 3′ sequencing primer ACCGGTGGATCCCGGG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR was a gift from Koen Breyne (Addgene plasmid # 207805 ; http://n2t.net/addgene:207805 ; RRID:Addgene_207805)

  • For your References section:

    Tracking human neurologic disease status in mouse brain/plasma using reporter-tagged, EV-associated biomarkers. Maalouf KE, Vaine CA, Frederick DM, Yoshinaga A, Obuchi W, Mahjoum S, Nieland L, Al Ali J, Bragg DC, Breakefield XO, Breyne K. Mol Ther. 2023 Jul 5;31(7):2206-2219. doi: 10.1016/j.ymthe.2023.05.011. Epub 2023 May 16. 10.1016/j.ymthe.2023.05.011 PubMed 37198883
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