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Addgene

MCP-mCherry-SEC61B
(Plasmid #207671)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207671 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SEC61B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1350
  • Entrez Gene
    SEC61B
  • Tag / Fusion Protein
    • MCP-mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BmtI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGHR
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.11.04.515216 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MCP-mCherry-SEC61B was a gift from Christine Mayr (Addgene plasmid # 207671 ; http://n2t.net/addgene:207671 ; RRID:Addgene_207671)

  • For your References section:

    Subcytoplasmic location of translation controls protein output. Horste EL, Fansler MM, Cai T, Chen X, Mitschka S, Zhen G, Lee FCY, Ule J, Mayr C. Mol Cell. 2023 Dec 21;83(24):4509-4523.e11. doi: 10.1016/j.molcel.2023.11.025. 10.1016/j.molcel.2023.11.025 PubMed 38134885
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