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Addgene

pAAV-hSyn1-FLEX-CybSEP2-WPRE
(Plasmid #207661)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207661 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-phSyn1(S)-FLEX-hGFP-T2A-SypeGFP-WPRE
  • Backbone size w/o insert (bp) 4434
  • Total vector size (bp) 6669
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cytochrome b-561
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    753
  • Mutation
    Histidine 86 and 159 to alanines, inserted 2 X SEP into 339-340 of Cyb561
  • GenBank ID
    NM_007805.5
  • Entrez Gene
    Cyb561
  • Promoter hSynapsin

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GCTGTACAGGTCTGCTTTGTATAGTTCATCCATGCCATG
  • 3′ sequencing primer GATGAACTATACAAAGACTACCACAAGAAGAAGGGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.01.19.524797 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn1-FLEX-CybSEP2-WPRE was a gift from Sung Han (Addgene plasmid # 207661 ; http://n2t.net/addgene:207661 ; RRID:Addgene_207661)

  • For your References section:

    Novel genetically encoded tools for imaging or silencing neuropeptide release from presynaptic terminals in vivo. Kim D-I, Park S, Ye M, Chen JY, Jhang J, Hunker AC, Zweifel LS, Palmiter RD, Han S. bioRxiv 2023.01.19.524797 10.1101/2023.01.19.524797
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