Skip to main content
Addgene

pS34.EF1α<GFP-2A-Cas9-RC
(Plasmid #207404)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207404 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pS34.EF1α
  • Backbone size w/o insert (bp) 4024
  • Total vector size (bp) 11302
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9-RC
  • Alt name
    eRad18-Cas9-CtIP
  • Species
    H. sapiens (human), Synthetic; S. pyogenes
  • Insert Size (bp)
    7278
  • Promoter EF1α
  • Tag / Fusion Protein
    • GFP-2A (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer cctcagacagtggttcaaagt
  • 3′ sequencing primer GAAATTTGTGATGCTATTGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2020.07.15.199620 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pS34.EF1α<GFP-2A-Cas9-RC was a gift from Alexandros Poulopoulos (Addgene plasmid # 207404 ; http://n2t.net/addgene:207404 ; RRID:Addgene_207404)

  • For your References section:

    Enhancing Precision and Efficiency of Cas9-Mediated Knockin Through Combinatorial Fusions of DNA Repair Proteins. Richardson RR, Steyert M, Khim SN, Crutcher GW, Brandenburg C, Robertson CD, Romanowski AJ, Inen J, Altas B, Poulopoulos A. CRISPR J. 2023 Oct;6(5):447-461. doi: 10.1089/crispr.2023.0036. Epub 2023 Sep 15. 10.1089/crispr.2023.0036 PubMed 37713292
Related items:
Commonly requested with: