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Addgene

pUltraHot-mCherry-BLM-WT
(Plasmid #206966)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206966 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUltraHot
  • Backbone size w/o insert (bp) 7468
  • Total vector size (bp) 12424
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Bloom Syndrome RecQ Like Helicase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4248
  • GenBank ID
    NM_000057.4
  • Entrez Gene
    BLM (a.k.a. BS, MGRISCE1, RECQ2, RECQL2, RECQL3)
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer tccaccggcggcatggacgagctgtacaagGCTGCTGTTCCTCAAAATAATCTAC
  • 3′ sequencing primer ctccggaacgaattctgatcacacgctagcTTATGAGAATGCATATGAAGGCTTAA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUltraHot-mCherry-BLM-WT was a gift from Eran Hornstein (Addgene plasmid # 206966 ; http://n2t.net/addgene:206966 ; RRID:Addgene_206966)

  • For your References section:

    BLM helicase protein negatively regulates stress granule formation through unwinding RNA G-quadruplex structures. Danino YM, Molitor L, Rosenbaum-Cohen T, Kaiser S, Cohen Y, Porat Z, Marmor-Kollet H, Katina C, Savidor A, Rotkopf R, Ben-Isaac E, Golani O, Levin Y, Monchaud D, Hickson ID, Hornstein E. Nucleic Acids Res. 2023 Jul 28:gkad613. doi: 10.1093/nar/gkad613. 10.1093/nar/gkad613 PubMed 37503837
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