pCAMBgfp
(Plasmid #206130)

• Purpose: This binary vector has been generated for introduction of SGFP in filamentous fungi using ATMT. Contains the hygromycin resistance gene and the SGFP gene.
• Depositing Lab: Anne Osbourn
• Publication: Sesma et al Nature. 2004 Sep 30;431(7008):582-6. doi: 10.1038/nature02880.

Backbone

• Vector backbone: pCAMBIA1300
• Backbone manufacturer: CAMBIA, Canberra, Australia
• Modifications to backbone: The plasmid has undergone rearrangements but the T-DNA (insert) remains intact. It should be released when digested in XhoI-EcoRI
• Vector type: binary vector for expression of SGFP in filamentous fungi
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: binary vector, this plasmid also replicates in Agrobacterium at 28 °C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: hygromycin resistance gene and the Promoter of ToxA+SGFP
• Insert Size (bp): 3400

Resource Information

• Supplemental Documents:
  • S6W_1_pCAMGFP_pLann.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid has rearranged but the T-DNA (insert) remains intact. It can be released when digested by XhoI-EcoRI.