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Addgene

EGFP Rab11a WT pcDNA3.1 Zeo (+)
(Plasmid #206079)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206079 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 Zeo(+)
  • Backbone manufacturer
    Invitrogen Life Technologies
  • Backbone size w/o insert (bp) 5015
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAB11A
  • Species
    H. sapiens (human)
  • Entrez Gene
    RAB11A (a.k.a. YL8)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CTGGCTAACTAGAGAACC
  • 3′ sequencing primer GTGTCAGCTCCGACTAG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Prof. T. Hebert

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP Rab11a WT pcDNA3.1 Zeo (+) was a gift from Annette Dolphin (Addgene plasmid # 206079 ; http://n2t.net/addgene:206079 ; RRID:Addgene_206079)

  • For your References section:

    The alpha2delta ligand gabapentin inhibits the Rab11-dependent recycling of the calcium channel subunit alpha2delta-2. Tran-Van-Minh A, Dolphin AC. J Neurosci. 2010 Sep 22;30(38):12856-67. doi: 10.1523/JNEUROSCI.2700-10.2010. 10.1523/JNEUROSCI.2700-10.2010 PubMed 20861389
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