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Addgene

ePPEmax*
(Plasmid #205244)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205244 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJIT163
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
  • Alt name
    nCas9-NC-MLV
  • Species
    Synthetic; ; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus
  • Insert Size (bp)
    6180
  • Mutation
    Detailed in manuscript

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ePPEmax* was a gift from Zong Yuan (Addgene plasmid # 205244 ; http://n2t.net/addgene:205244 ; RRID:Addgene_205244)

  • For your References section:

    Efficient and versatile multiplex prime editing in hexaploid wheat. Ni P, Zhao Y, Zhou X, Liu Z, Huang Z, Ni Z, Sun Q, Zong Y. Genome Biol. 2023 Jun 29;24(1):156. doi: 10.1186/s13059-023-02990-1. 10.1186/s13059-023-02990-1 PubMed 37386475
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