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pE-35S-miniSdd7-nCas9-PBE-GmPPO2
(Plasmid #204855)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204855 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSE901
  • Backbone manufacturer
    Qi-jun Chen
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    EPSPS

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miniSdd7-nSpCas9(D10A)-UGI-mScarlet
  • Alt name
    soybean PPO2 sgRNA
  • Species
    Synthetic
  • Mutation
    D10A in SpCas9
  • Promoter 35S, AtU6-26p

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.05.21.541555 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pE-35S-miniSdd7-nCas9-PBE-GmPPO2 was a gift from Caixia Gao (Addgene plasmid # 204855 ; http://n2t.net/addgene:204855 ; RRID:Addgene_204855)

  • For your References section:

    Discovery of deaminase functions by structure-based protein clustering. Huang J, Lin Q, Fei H, He Z, Xu H, Li Y, Qu K, Han P, Gao Q, Li B, Liu G, Zhang L, Hu J, Zhang R, Zuo E, Luo Y, Ran Y, Qiu JL, Zhao KT, Gao C. Cell. 2023 Jul 20;186(15):3182-3195.e14. doi: 10.1016/j.cell.2023.05.041. Epub 2023 Jun 27. 10.1016/j.cell.2023.05.041 PubMed 37379837
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