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pACS145
(Plasmid #204600)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204600 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLENTI6.3/V5 -TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7692
  • Total vector size (bp) 8101
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    histone H3.3 K36M
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    410
  • Mutation
    histone H3.3 K36M
  • GenBank ID
    3020
  • Entrez Gene
    H3-3A (a.k.a. BRYLIB1, H3-3B, H3.3A, H3F3, H3F3A)
  • Promoter CMV
  • Tag / Fusion Protein
    • human histone H3.3 K36M - V5 (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACS145 was a gift from Tom Misteli (Addgene plasmid # 204600 ; http://n2t.net/addgene:204600 ; RRID:Addgene_204600)

  • For your References section:

    Identification of epigenetic modulators as determinants of nuclear size and shape. Schibler AC, Jevtic P, Pegoraro G, Levy DL, Misteli T. Elife. 2023 May 23;12:e80653. doi: 10.7554/eLife.80653. 10.7554/eLife.80653 PubMed 37219077
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