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pIVM_26
(Plasmid #204501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204501 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDF-1b DUET
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3781
  • Total vector size (bp) 4540
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21 or Rosetta and drop temperature to 23°C for expression of protein
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EloB (1-104) and EloC (17-112)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    757
  • Mutation
    0
  • GenBank ID
    NP_009039.1 (EloB) NP_001191792 (EloC)
  • Entrez Gene
    ELOC (a.k.a. SIII, TCEB1)
  • Entrez Gene
    ELOB (a.k.a. SIII, TCEB2)
  • Promoter AmpR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco1 (EloB) and Nde1 (EloC) (not destroyed)
  • 3′ cloning site HindIII (EloB) and Xho1 (EloC) (not destroyed)
  • 5′ sequencing primer DUET primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gadd MS, Testa A, Lucas X, Chan KH, Chen W, Lamont DJ, Zengerle M, Ciulli A. Structural basis of PROTAC cooperative recognition for selective protein degradation. Nat Chem Biol. 2017 May;13(5):514-521. doi: 10.1038/nchembio.2329. Epub 2017 Mar 13. PMID: 28288108; PMCID: PMC5392356.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIVM_26 was a gift from Alessio Ciulli (Addgene plasmid # 204501 ; http://n2t.net/addgene:204501 ; RRID:Addgene_204501)

  • For your References section:

    Crystal Structure of the Cul2-Rbx1-EloBC-VHL Ubiquitin Ligase Complex. Cardote TAF, Gadd MS, Ciulli A. Structure. 2017 Jun 6;25(6):901-911.e3. doi: 10.1016/j.str.2017.04.009. 10.1016/j.str.2017.04.009 PubMed 28591624
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