PB-CAG-rtTA-IRES-eGFP-pGK-NeoR
(Plasmid
#204497)
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PurposeExpresses rtTA and Neomycin resistance genes in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 204497 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namertTA
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-CAG-rtTA-IRES-eGFP-pGK-NeoR was a gift from Wataru Fujii (Addgene plasmid # 204497 ; http://n2t.net/addgene:204497 ; RRID:Addgene_204497) -
For your References section:
Establishment of African pygmy mouse induced pluripotent stem cells using defined doxycycline inducible transcription factors. Matsuya S, Fujino K, Imai H, Kusakabe KT, Fujii W, Kano K. Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.1038/s41598-024-53687-9. 10.1038/s41598-024-53687-9 PubMed 38331995