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Addgene

pCMV-SARS-CoV-2 Nsp3TMC111A
(Plasmid #204207)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204207 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Tag2B
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/)
  • Species
    Other
  • Mutation
    Cys to Ala in active site of PLpro
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Tag / Fusion Protein
    • FLAGx1 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Amino acids 742-1579 of Nsp3, mutation of aa111 of PLpro (aa866 of Addgene clone 141257).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-SARS-CoV-2 Nsp3TMC111A was a gift from Allan Weissman (Addgene plasmid # 204207 ; http://n2t.net/addgene:204207 ; RRID:Addgene_204207)

  • For your References section:

    SARS-CoV-2 papain-like protease plays multiple roles in regulating cellular proteins in the endoplasmic reticulum. Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, Loncarek J, Tsai YC, Weissman AM. J Biol Chem. 2023 Oct 12;299(12):105346. doi: 10.1016/j.jbc.2023.105346. 10.1016/j.jbc.2023.105346 PubMed 37838170
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