CMV5.1-SARS-CoV-2 Nsp3
(Plasmid
#204205)
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PurposeMammalian expression of SARS-CoV-2 Nsp3
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 204205 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCMV5.1p
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/)
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SpeciesOther
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Mutationnone, from obtained clone
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Entrez GeneORF1ab (a.k.a. GU280_gp01)
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Tag
/ Fusion Protein
- FLAGx3 (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' Cloning Site: ClaI (not destroyed), 3' Cloning Site: NotI (not destroyed). Contains entire SARS-CoV-2 Nsp3 from Wuhan isolate.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV5.1-SARS-CoV-2 Nsp3 was a gift from Allan Weissman (Addgene plasmid # 204205 ; http://n2t.net/addgene:204205 ; RRID:Addgene_204205) -
For your References section:
SARS-CoV-2 papain-like protease plays multiple roles in regulating cellular proteins in the endoplasmic reticulum. Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, Loncarek J, Tsai YC, Weissman AM. J Biol Chem. 2023 Oct 12;299(12):105346. doi: 10.1016/j.jbc.2023.105346. 10.1016/j.jbc.2023.105346 PubMed 37838170
