mEos3.2GPI
(Plasmid #203759)

• Purpose: Encodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.
• Depositing Lab: Sarah Veatch
• Publication: Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. doi: 10.1038/s41589-023-01268-8. Epub 2023 Mar 30.

Backbone

• Vector backbone: mEos3.2-C1
• Backbone manufacturer: Addgene; Michael Davidson's lab
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CD58 c-term
• Species: H. sapiens (human)
• Entrez Gene: CD58 (a.k.a. LFA-3, LFA3, ag3)
• Tag / Fusion Protein:
  • mEos3.2 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • mEos3.2GPI.gb
• A portion of this plasmid was derived from a plasmid made by: Insert DNA courtesy of the Kai Simons lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry