mEos3.2GG
(Plasmid #203756)

• Purpose: Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.
• Depositing Lab: Sarah Veatch
• Publication: Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. doi: 10.1038/s41589-023-01268-8. Epub 2023 Mar 30.

Backbone

• Vector backbone: mEos3.2-C1
• Backbone manufacturer: Addgene; Michael Davidson's lab
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KRas c-term
• Species: H. sapiens (human)
• Entrez Gene: KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
• Tag / Fusion Protein:
  • mEos3.2 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • mEos3.2GG.gb
• A portion of this plasmid was derived from a plasmid made by: Insert DNA courtesy of the Barbara Baird and David Holowka lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry