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Addgene

pGEX4T-1-RUNX3 aa 49-187
(Plasmid #203440)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203440 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T1
  • Backbone manufacturer
    Amersham
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RUNX3
  • Species
    H. sapiens (human)
  • Entrez Gene
    RUNX3 (a.k.a. AML2, CBFA3, PEBP2aC)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer pGEX5
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T-1-RUNX3 aa 49-187 was a gift from Yoshiaki Ito (Addgene plasmid # 203440 ; http://n2t.net/addgene:203440 ; RRID:Addgene_203440)

  • For your References section:

    RUNX3 inactivates oncogenic MYC through disruption of MYC/MAX complex and subsequent recruitment of GSK3beta-FBXW7 cascade. Oei V, Chuang LSH, Matsuo J, Srivastava S, Teh M, Ito Y. Commun Biol. 2023 Jul 3;6(1):689. doi: 10.1038/s42003-023-05037-0. 10.1038/s42003-023-05037-0 PubMed 37400551
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