TetO-FUW-OSKM Notes
Addgene diagnostic digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: XhoI.
We expected the following band sizes for this digest: 1.45kb, 2kb, 2.6kb and 7.3kb. We see the expected pattern.
Addgene XhoI and SacII diagnostic digests
This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: XhoI and SacII.
We expected the following band sizes for the XhoI digest: 1.45kb, 2kb, 2.6kb and 7.3kb. We see the expected pattern.
We expected the following band sizes for the SacII digest: 1.35kb, 1.5kb, 2.8kb and 7.8kb. We see the expected pattern.
