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pRSF-DUET1-DHDDS/Sumo-NgBR
(Plasmid #203162)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203162 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF-DUET1
  • Backbone manufacturer
    Novagene
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    DHDDS
  • Species
    H. sapiens (human)
  • Entrez Gene
    DHDDS (a.k.a. CIT, CPT, DEDSM, DS, HDS, RP59, hCIT)

Gene/Insert 2

  • Gene/Insert name
    NUS1 dehydrodolichyl diphosphate synthase subunit
  • Alt name
    NgBR
  • Species
    H. sapiens (human)
  • Mutation
    truncation
  • Entrez Gene
    NUS1 (a.k.a. C6orf68, CDG1AA, MGC:7199, MRD55, NgBR, TANGO14)
  • Tag / Fusion Protein
    • His6-SUMO (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSF-DUET1-DHDDS/Sumo-NgBR was a gift from William Sessa (Addgene plasmid # 203162 ; http://n2t.net/addgene:203162 ; RRID:Addgene_203162)

  • For your References section:

    Structural elucidation of the cis-prenyltransferase NgBR/DHDDS complex reveals insights in regulation of protein glycosylation. Edani BH, Grabinska KA, Zhang R, Park EJ, Siciliano B, Surmacz L, Ha Y, Sessa WC. Proc Natl Acad Sci U S A. 2020 Aug 25;117(34):20794-20802. doi: 10.1073/pnas.2008381117. Epub 2020 Aug 12. 10.1073/pnas.2008381117 PubMed 32817466
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