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Addgene

pNuc-cis
(Plasmid #202796)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202796 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTA-Mob
  • Backbone size w/o insert (bp) 52707
  • Total vector size (bp) 61203
  • Vector type
    Bacterial Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TevSpCas9, sgRNA cassette, oriT, CenArsHis
  • Insert Size (bp)
    8500

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNuc-cis was a gift from David Edgell (Addgene plasmid # 202796 ; http://n2t.net/addgene:202796 ; RRID:Addgene_202796)

  • For your References section:

    Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing. Hamilton TA, Pellegrino GM, Therrien JA, Ham DT, Bartlett PC, Karas BJ, Gloor GB, Edgell DR. Nat Commun. 2019 Oct 4;10(1):4544. doi: 10.1038/s41467-019-12448-3. 10.1038/s41467-019-12448-3 PubMed 31586051
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