pBbS5c-mCherry-AsLOV2*(543)
(Plasmid
#202712)
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PurposeExpresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202712 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBbS5c-mCherry
- Backbone size w/o insert (bp) 5617
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemCherry-AsLOV2*(543)
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SpeciesSynthetic
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Insert Size (bp)1146
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.02.25.530042v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBbS5c-mCherry-AsLOV2*(543) was a gift from Mary Dunlop (Addgene plasmid # 202712 ; http://n2t.net/addgene:202712 ; RRID:Addgene_202712) -
For your References section:
Light-inducible protein degradation in E. coli with the LOVdeg tag. Tague N, Coriano-Ortiz C, Sheets MB, Dunlop MJ. Elife. 2024 Jan 25;12:RP87303. doi: 10.7554/eLife.87303. 10.7554/eLife.87303 PubMed 38270583
