pAAV-EF1a-DIO-mCherry-WPRE
(Plasmid #202620)

• Purpose: Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)
• Depositing Lab: Francois St-Pierre
• Publication: Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi: 10.1038/s41467-023-41975-3.

Backbone

• Vector backbone: pAAV-EF1a-DIO
• Backbone size w/o insert (bp): 5611
• Total vector size (bp): 6325
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry
• Species: Discosoma sp.
• Insert Size (bp): 714
• Promoter: EF1a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: TCAAGCCTCAGACAGTGGTTC
• 3′ sequencing primer: GGTTGATTATCGATAAGCTTGATATCG

Resource Information

• Supplemental Documents:
  • paav-ef1a-dio-mcherry-wpre.gb
• A portion of this plasmid was derived from a plasmid made by: https://doi.org/10.1038/nbt1037

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2022.08.29.505018 for bioRxiv preprint.