pOTTC1664 - pAAV SYN1 mGas6(delta)-Myc-DDK
(Plasmid #202541)

• Purpose: An adeno-associated viral vector expressing murine Gas6 with deletion of (F50-E275) fused Myc and DDK epitopes from a synapsin promoter
• Depositing Lab: Christopher Richie
• Publication: Owlett et al J Neuroinflammation. 2022 Feb 7;19(1):38. doi: 10.1186/s12974-022-02397-y.

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Backbone

• Vector backbone: pOTTC1479
• Backbone manufacturer: NIDA GEVVC
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Gas6
• Species: M. musculus (mouse)
• Insert Size (bp): 1440
• Mutation: Deletion of F50-E275
• Entrez Gene: Gas6 (a.k.a. Gas-6)
• Promoter: SYN1
• Tag / Fusion Protein:
  • Myc-DDK (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning

Resource Information

• Supplemental Documents:
  • pOTTC1664 - pAAV SYN1 Gas6(dEGFR)-Myc-DDK.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry