pOTTC1664 - pAAV SYN1 mGas6(delta)-Myc-DDK
(Plasmid
#202541)
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PurposeAn adeno-associated viral vector expressing murine Gas6 with deletion of (F50-E275) fused Myc and DDK epitopes from a synapsin promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202541 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOTTC1479
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Backbone manufacturerNIDA GEVVC
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGas6
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SpeciesM. musculus (mouse)
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Insert Size (bp)1440
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MutationDeletion of F50-E275
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Entrez GeneGas6 (a.k.a. Gas-6)
- Promoter SYN1
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Tag
/ Fusion Protein
- Myc-DDK (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOTTC1664 - pAAV SYN1 mGas6(delta)-Myc-DDK was a gift from Christopher Richie (Addgene plasmid # 202541 ; http://n2t.net/addgene:202541 ; RRID:Addgene_202541) -
For your References section:
Gas6 induces inflammation and reduces plaque burden but worsens behavior in a sex-dependent manner in the APP/PS1 model of Alzheimer's disease. Owlett LD, Karaahmet B, Le L, Belcher EK, Dionisio-Santos D, Olschowka JA, Elliott MR, O'Banion MK. J Neuroinflammation. 2022 Feb 7;19(1):38. doi: 10.1186/s12974-022-02397-y. 10.1186/s12974-022-02397-y PubMed 35130912