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Addgene

pCDNA3/GFP-PR
(Plasmid #20253)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20253 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIV-1 protease
  • Alt name
    HIV
  • Alt name
    PR
  • Species
    human immunodeficiency virus type 1
  • Insert Size (bp)
    6563
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3/GFP-PR was a gift from Nico Dantuma (Addgene plasmid # 20253 ; http://n2t.net/addgene:20253 ; RRID:Addgene_20253)

  • For your References section:

    Cell-based fluorescence assay for human immunodeficiency virus type 1 protease activity. Lindsten K, Uhlikova T, Konvalinka J, Masucci MG, Dantuma NP. Antimicrob Agents Chemother. 2001 Sep . 45(9):2616-22. 10.1128/AAC.45.9.2616-2622.2001 PubMed 11502538
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