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Addgene

pYS16
(Plasmid #202082)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202082 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MN100A-1
  • Backbone manufacturer
    System Biosciences
  • Vector type
    CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CT mNG-T2A-PuroR Minicircle Parent
  • Species
    Synthetic
  • Promoter None
  • Tag / Fusion Protein
    • mNeonGreen (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

IMPORTANT: This plasmid is shipped from Addgene in NEB Stable cells, but these cells are NOT suitable for minicircle production. You will need to purchase ZYCY10P3S2T cells separately, and transform the plasmid into that host strain, to produce minicircles.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYS16 was a gift from Daniel Dickinson (Addgene plasmid # 202082 ; http://n2t.net/addgene:202082 ; RRID:Addgene_202082)

  • For your References section:

    Efficient and rapid fluorescent protein knock-in with universal donors in mouse embryonic stem cells. Shi Y, Kopparapu N, Ohler L, Dickinson DJ. Development. 2023 May 15;150(10):dev201367. doi: 10.1242/dev.201367. Epub 2023 May 25. 10.1242/dev.201367 PubMed 37129004
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