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Addgene

pDD428
(Plasmid #202081)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202081 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRISPAINT
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pEF1a-mCherry-Cas9 + Myh9 CT sgRNA
  • gRNA/shRNA sequence
    Ggtcgacggtaaagcagatg
  • Species
    S. pyogenes (Cas9)
  • Entrez Gene
    Myh9 (a.k.a. Fltn, Myhn-1, Myhn1, NMHC II-A, NMHCIIA, NMMHC-A, NMMHC-IIA, TU72.6)
  • Promoter EF1a

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDD428 was a gift from Daniel Dickinson (Addgene plasmid # 202081 ; http://n2t.net/addgene:202081 ; RRID:Addgene_202081)

  • For your References section:

    Efficient and rapid fluorescent protein knock-in with universal donors in mouse embryonic stem cells. Shi Y, Kopparapu N, Ohler L, Dickinson DJ. Development. 2023 May 15;150(10):dev201367. doi: 10.1242/dev.201367. Epub 2023 May 25. 10.1242/dev.201367 PubMed 37129004
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