pBEL2836
(Plasmid
#201997)
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PurposeComplementation of M. smegmatis LucB(WT)
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201997 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMV261-Zeo
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namelucB
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Alt nameMSMEG_3032
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SpeciesM. smegmatis
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer cagcgaggacaacttgag
- 3′ sequencing primer tgtcaacacgtgctcgag (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byJeffery Cox (source of pMV261-Zeo plasmid)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.12.08.519548v2.full for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBEL2836 was a gift from Gira Bhabha & Damian Ekiert (Addgene plasmid # 201997 ; http://n2t.net/addgene:201997 ; RRID:Addgene_201997) -
For your References section:
Structure of an endogenous mycobacterial MCE lipid transporter. Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert D. Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi: 10.21203/rs.3.rs-2412186/v1. Preprint. 10.21203/rs.3.rs-2412186/v1 PubMed 36711512
