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Addgene

pJB94
(Plasmid #208752)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208752 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pJB06 (Addgene #190480)
  • Total vector size (bp) 8780

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grown on sucrose-containing plates will counter select cells that contain the sacB insert
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sacB
  • Alt name
    levansucrase
  • Insert Size (bp)
    1422
  • Entrez Gene
    sacB (a.k.a. BSU_34450, BSU34450)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJB94 was a gift from Joseph Sorg (Addgene plasmid # 208752 ; http://n2t.net/addgene:208752 ; RRID:Addgene_208752)
  • For your References section:

    Theophylline-based control of repA on a Clostridioides difficile plasmid for use in allelic exchange. Brehm JN, Sorg JA. Anaerobe. 2024 Apr 29;88:102858. doi: 10.1016/j.anaerobe.2024.102858. 10.1016/j.anaerobe.2024.102858 PubMed 38692475