pJB94
(Plasmid
#208752)
-
PurposeTheophylline-based control of repA on a C. difficile plasmid for use in allelic exchange
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 208752 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepJB06 (Addgene #190480)
- Total vector size (bp) 8780
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsGrown on sucrose-containing plates will counter select cells that contain the sacB insert
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namesacB
-
Alt namelevansucrase
-
Insert Size (bp)1422
-
Entrez GenesacB (a.k.a. BSU_34450, BSU34450)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pJB94 was a gift from Joseph Sorg (Addgene plasmid # 208752 ; http://n2t.net/addgene:208752 ; RRID:Addgene_208752) -
For your References section:
Theophylline-based control of repA on a Clostridioides difficile plasmid for use in allelic exchange. Brehm JN, Sorg JA. Anaerobe. 2024 Apr 29;88:102858. doi: 10.1016/j.anaerobe.2024.102858. 10.1016/j.anaerobe.2024.102858 PubMed 38692475