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Addgene

h3.7SP-Crtta
(Plasmid #20180)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20180 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 2700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    h3.7SP-Crtta
  • Alt name
    Sftpc-reverse tetracycline transactivator
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5100
  • Entrez Gene
    SFTPC (a.k.a. BRICD6, PSP-C, SFTP2, SMDP2, SP-C, SP5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer M13_forward20
  • 3′ sequencing primer M13rev
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    We cloned the promoter (3.7SP-C), Tet Systems the rtTA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Used to express genes in the lung epithelium or lung epithelial cells

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    h3.7SP-Crtta was a gift from Jeffrey Whitsett (Addgene plasmid # 20180 ; http://n2t.net/addgene:20180 ; RRID:Addgene_20180)

  • For your References section:

    Conditional expression of fibroblast growth factor-7 in the developing and mature lung. Tichelaar JW, Lu W, Whitsett JA. J Biol Chem. 2000 Apr 21. 275(16):11858-64. 10.1074/jbc.275.16.11858 PubMed 10766812
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