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Addgene

CC10-rtTA
(Plasmid #20179)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20179 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II KS+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    rcc10 promoter
  • Alt name
    ccsp-rtTA
  • Species
    R. norvegicus (rat)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 Fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The expression vector pCC10/NrtTA/hGH was made by ligating the following gene fragments into pBIIKS(+):
1) 2.3kb HindIII fragment of rat CC10 promoter
2) 1.0kb HindIII/BamHI fragment of NrtTA coding seq
3) 2.0kb BamHI/NotI fragment of human growth hormone gene sequence extending from the BamHI site in the first exon to the EcoRI site after the poly-A addition signal.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CC10-rtTA was a gift from Jeffrey Whitsett (Addgene plasmid # 20179 ; http://n2t.net/addgene:20179 ; RRID:Addgene_20179)

  • For your References section:

    cis-acting elements that confer lung epithelial cell expression of the CC10 gene. Stripp BR, Sawaya PL, Luse DS, Wikenheiser KA, Wert SE, Huffman JA, Lattier DL, Singh G, Katyal SL, Whitsett JA. J Biol Chem. 1992 Jul 25. 267(21):14703-12. PubMed 1634515
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