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Addgene

PrimeRoot.v2C-FLP
(Plasmid #201740)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201740 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJIT163
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ePPE,FLP
  • Alt name
    enhanced plant prime editor
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PrimeRoot.v2C-FLP was a gift from Caixia Gao (Addgene plasmid # 201740 ; http://n2t.net/addgene:201740 ; RRID:Addgene_201740)

  • For your References section:

    Precise integration of large DNA sequences in plant genomes using PrimeRoot editors. Sun C, Lei Y, Li B, Gao Q, Li Y, Cao W, Yang C, Li H, Wang Z, Li Y, Wang Y, Liu J, Zhao KT, Gao C. Nat Biotechnol. 2023 Apr 24. doi: 10.1038/s41587-023-01769-w. 10.1038/s41587-023-01769-w PubMed 37095350
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