PrimeRoot.v2C-Cre
(Plasmid
#201739)
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PurposeFor DNA insertion test in protoplast
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201739 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJIT163
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameePPE,Cre
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Alt nameenhanced plant prime editor
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PrimeRoot.v2C-Cre was a gift from Caixia Gao (Addgene plasmid # 201739 ; http://n2t.net/addgene:201739 ; RRID:Addgene_201739) -
For your References section:
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors. Sun C, Lei Y, Li B, Gao Q, Li Y, Cao W, Yang C, Li H, Wang Z, Li Y, Wang Y, Liu J, Zhao KT, Gao C. Nat Biotechnol. 2023 Apr 24. doi: 10.1038/s41587-023-01769-w. 10.1038/s41587-023-01769-w PubMed 37095350