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Addgene

EYFP-Rab7
(Plasmid #20164)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20164 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEYFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha, HB101
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rab7
  • Species
    Canis lupus familiaris
  • Insert Size (bp)
    621
  • GenBank ID
    NP_001003316
  • Entrez Gene
    RAB7A (a.k.a. RAB7)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Suzanne Pfeffer (Stanford Med School)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EYFP-Rab7 was a gift from Xiaowei Zhuang (Addgene plasmid # 20164 ; http://n2t.net/addgene:20164 ; RRID:Addgene_20164)

  • For your References section:

    Ligands for clathrin-mediated endocytosis are differentially sorted into distinct populations of early endosomes. Lakadamyali M, Rust MJ, Zhuang X. Cell. 2006 Mar 10. 124(5):997-1009. 10.1016/j.cell.2005.12.038 PubMed 16530046
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