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pGL4 mIL-17 2kb promoter + CNS-5
(Plasmid #20128)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20128 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.10
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4242
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mIL-17
  • Alt name
    mIL-17 promoter + enhancer (CNS-5)
  • Alt name
    CNS-5
  • Alt name
    IL-17
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • GenBank ID
    AC159614
  • Entrez Gene
    Il17a (a.k.a. Ctla-8, Ctla8, IL-17, IL-17A, Il17)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site BglII (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4 mIL-17 2kb promoter + CNS-5 was a gift from Warren Strober (Addgene plasmid # 20128 ; http://n2t.net/addgene:20128 ; RRID:Addgene_20128)

  • For your References section:

    Interactions between the transcription factors Runx1, RORgt and Foxp3 regulate differentiation of interleukin 17-producing T cells. Fuping Zhang, Guangxun Meng & Warren Strober. 2008 Nov;9(11):1297-306. Epub 2008 Oct 12. 10.1038/ni.1663 PubMed 18849990
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