SuExp His-Myc-mPLD4
(Plasmid #201185)

• Purpose: Protein expression plasmid for recombinant His-Myc tag mouse PLD4
• Depositing Lab: David Nemazee
• Publication: Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi: 10.1038/s41467-021-26150-w.

Backbone

• Vector backbone: SuExp
• Backbone size w/o insert (bp): 2000
• Total vector size (bp): 3500
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PLD4
• Species: M. musculus (mouse)
• Insert Size (bp): 1500
• Mutation: cytosolic and transmembrane domain truncated; add a signal peptide for secretion
• GenBank ID: 104759
• Entrez Gene: Pld4 (a.k.a. thss)
• Entrez Gene: Pld4
• Entrez Gene: Pld4 (a.k.a. Sam-9)
• Promoter: CMV
• Tag / Fusion Protein:
  • His-Myc, Factor Xa cleavable (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry