pDUET-ctCPR-sAMO
(Plasmid
#20111)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20111 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepETDuet-1
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5420
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namep450 reductase and codon optimized amorphadiene oxidase
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Alt nameCPR and AMO
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Speciesc. tropicalis and a. annua
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDUET-ctCPR-sAMO was a gift from Jay Keasling (Addgene plasmid # 20111 ; http://n2t.net/addgene:20111 ; RRID:Addgene_20111) -
For your References section:
Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Chang MC, Eachus RA, Trieu W, Ro DK, Keasling JD. Nat Chem Biol. 2007 May . 3(5):274-7. 10.1038/nchembio875 PubMed 17438551