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Addgene

AAV-cfGFP
(Plasmid #200773)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200773 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-Syn_MCS_WPRE_Sv40pA
  • Backbone manufacturer
    VectorBuilder
  • Backbone size w/o insert (bp) 4949
  • Total vector size (bp) 5663
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cysteine-free GFP
  • Alt name
    cfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter Synapsin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GCCGCCTCAGCACTGAAG
  • 3′ sequencing primer GCAGCGTATCCACATAGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The cfGFP plasmid equivalent to Addgene #37533 was a kind gift from Dr. Ikuo Wada.

Please note: The plasmid contains a 22bp deletion in the 5' ITR. This deletion is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-cfGFP was a gift from Alexander Dityatev (Addgene plasmid # 200773 ; http://n2t.net/addgene:200773 ; RRID:Addgene_200773)

  • For your References section:

    A shift in the mechanisms controlling hippocampal engram formation during brain maturation. Ramsaran AI, Wang Y, Golbabaei A, Aleshin S, de Snoo ML, Yeung BA, Rashid AJ, Awasthi A, Lau J, Tran LM, Ko SY, Abegg A, Duan LC, McKenzie C, Gallucci J, Ahmed M, Kaushik R, Dityatev A, Josselyn SA, Frankland PW. Science. 2023 May 5;380(6644):543-551. doi: 10.1126/science.ade6530. Epub 2023 May 4. 10.1126/science.ade6530 PubMed 37141366
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