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Addgene

pSX2720
(Plasmid #200764)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200764 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR8
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    scFv-sfGFP
  • Alt name
    scFv-sfGFP
  • Species
    C. elegans (nematode)
  • Promoter col-19

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ggtacaggtagagctcaccggtgcaaccatgggccccgac
  • 3′ sequencing primer gctgggtcgaattcgcccttttactctagactcgagcggc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSX2720 was a gift from Suhong Xu (Addgene plasmid # 200764 ; http://n2t.net/addgene:200764 ; RRID:Addgene_200764)

  • For your References section:

    Enhanced single RNA imaging reveals dynamic gene expression in live animals. Hu Y, Xu J, Gao E, Fan X, Wei J, Ye B, Xu S, Ma W. Elife. 2023 Mar 3;12:e82178. doi: 10.7554/eLife.82178. 10.7554/eLife.82178 PubMed 36867026
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