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Addgene

pSX2719
(Plasmid #200763)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200763 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Psemo-1
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MCP-24xGCN4
  • Species
    C. elegans (nematode)
  • Promoter semo-1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tcacaagtttgtacaaaaaagcaggctatggcttctaactttactcagttcg
  • 3′ sequencing primer gtgccgccaagaaaaaagtctcattaacccgagccagaaccc
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSX2719 was a gift from Suhong Xu (Addgene plasmid # 200763 ; http://n2t.net/addgene:200763 ; RRID:Addgene_200763)

  • For your References section:

    Enhanced single RNA imaging reveals dynamic gene expression in live animals. Hu Y, Xu J, Gao E, Fan X, Wei J, Ye B, Xu S, Ma W. Elife. 2023 Mar 3;12:e82178. doi: 10.7554/eLife.82178. 10.7554/eLife.82178 PubMed 36867026
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