pIA034
(Plasmid #200421)

• Purpose: (Empty Backbone) amyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.
• Depositing Lab: Rut Carballido-López
• Publication: Altinoglu et al bioRxiv 2023

Backbone

• Vector backbone: pDG364
• Backbone size (bp): 6330
• Vector type: Bacterial Expression
• Selectable markers: Chloramphenicol
• Tag / Fusion Protein:
  • bsdronPA (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: E. coli: 37°C and selection Ampicillin 100 μg/mL B. subtilis: 30°C and selection Chloramphenicol 5 μg/mL
• Copy number: Low Copy

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pIA034 aka AmyE-x-bsDronPA.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

bsdronPA was amplified from pIA019 using primers oIA054 and oIA055, then cloned into pIA021 digested with BamHI and HindIII, giving plasmid pIA034, amyE::(bsdronPA cm).