Skip to main content
Addgene

pLenti-SF3B1-K700E-reporter
(Plasmid #200318)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200318 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLenti
  • Total vector size (bp) 7538
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BSD-2A-EGFPi
  • Alt name
    LoxP-WPRE-EF1a-BSD-P2A-EGFP(exon 1)-synMTERFD3i1-150-EGFP(exon 2)-LoxP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2298
  • Promoter EF1-a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Whole plasmid sequencing with Primordium.
  • 3′ sequencing primer Whole plasmid sequencing with Primordium.
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.23.581842 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-SF3B1-K700E-reporter was a gift from Lili Wang (Addgene plasmid # 200318 ; http://n2t.net/addgene:200318 ; RRID:Addgene_200318)

  • For your References section:

    Engineering oncogenic hotspot mutations on SF3B1 via CRISPR-directed PRECIS mutagenesis. Fernandez M, Yu L, Jia Q, Wang X, Hart KL, Jia Z, Lin RJ, Wang L. Cancer Res Commun. 2024 Aug 28. doi: 10.1158/2767-9764.CRC-24-0145. 10.1158/2767-9764.CRC-24-0145 PubMed 39194178
Related items:
Commonly requested with: