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Addgene

SUMO_LPETG_MT3
(Plasmid #200304)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200304 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHYRSF53
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    metallothionein 3
  • Species
    H. sapiens (human)
  • Entrez Gene
    MT3 (a.k.a. GIF, GIFB, GRIF, ZnMT3)
  • Promoter T7
  • Tag / Fusion Protein
    • Smt3 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SUMO_LPETG_MT3 was a gift from Artur Krężel (Addgene plasmid # 200304 ; http://n2t.net/addgene:200304 ; RRID:Addgene_200304)

  • For your References section:

    Calcium-assisted sortase A cleavage of SUMOylated metallothionein constructs leads to high-yield production of human MT3. Singh AK, Krezel A. Microb Cell Fact. 2023 Jul 11;22(1):125. doi: 10.1186/s12934-023-02134-x. 10.1186/s12934-023-02134-x PubMed 37434134
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