pDEST12.2 DYRK2 WT
(Plasmid #20005)

• Depositing Lab: Anjana Rao
• Publication: Gwack et al Nature. 2006 Jun 1. 441(7093):646-50.

Backbone

• Vector backbone: pDEST12.2
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DYRK2
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• Entrez Gene: DYRK2
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Dyrk2 pDEST12.2 contains the full cDNA of Dyrk2 (sequence of cDNA attached) cloned Not1/Xho1 into pENTR.11 then transferred by recombination into pDEST12.2 (Gateway). It has an N-terminal FLAG tag, which was put directly into pENTR.11, but the Not1/Xho1 cut will excise the dyrk2 cdna without the tag (but with an atg and a stop codon as well); the size is 1806 bp.