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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 19982 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTYF
- Backbone size w/o insert (bp) 7406
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructions30C
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name2xSYN-tTA
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SpeciesH. sapiens (human), S. cerevisiae (budding yeast); E.coli
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Insert Size (bp)3683
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Entrez GeneSYN1 (a.k.a. EPILX, EPILX1, MRX50, SYN1a, SYN1b, SYNI)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer GTAGACATAA TAGCAACAGA C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTYF-2xSYN-tTA was a gift from Sergey Kasparov (Addgene plasmid # 19982 ; http://n2t.net/addgene:19982 ; RRID:Addgene_19982) -
For your References section:
Enhancement of cell-specific transgene expression from a Tet-Off regulatory system using a transcriptional amplification strategy in the rat brain. Liu B, Wang S, Brenner M, Paton JF, Kasparov S. J Gene Med. 2008 May . 10(5):583-92. 10.1002/jgm.1178 PubMed 18324737
Map uploaded by the depositor.
