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pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO
(Plasmid #199781)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199781 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T1
  • Backbone size w/o insert (bp) 5710
  • Total vector size (bp) 6967
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NEMO
  • Alt name
    IKBKG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1257
  • GenBank ID
    AF062089.1
  • Entrez Gene
    IKBKG (a.k.a. AMCBX1, EDAID1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, SAIDX, ZC2HC9)
  • Tag / Fusion Protein
    • GST-Thrombin-TEV-EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer aataggcacctctggaagagccaactgtgt
  • 3′ sequencing primer ctactcaatgcactccatgacatgtatctg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO was a gift from Sascha Martens (Addgene plasmid # 199781 ; http://n2t.net/addgene:199781 ; RRID:Addgene_199781)
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