pAAV_CMV::V5-EGFR
(Plasmid #199717)

• Purpose: Expresses V5-tagged EGFR in mammalian cells.
• Depositing Lab: Alice Ting
• Publication: Roh et al J Am Chem Soc. 2023 May 10;145(18):10220-10226. doi: 10.1021/jacs.3c01145. Epub 2023 Apr 26.

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Backbone

• Vector backbone: pAAV
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: V5-EGFR
• Species: H. sapiens (human), Synthetic
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • V5-EGFR_09.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.03.03.531050 for bioRxiv preprint. In the original study XL1 Blue E. coli are used for amplifying plasmids.