pET12a-PAPI
(Plasmid #19961)

• Depositing Lab: Jonathan Tait
• Publication: Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

Backbone

• Vector backbone: pET12a
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 4606
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21(DE3)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Annexin V
• Alt name: Annexin A5
• Alt name: Placental anticoagulant protein I
• Alt name: Endonexin II
• Species: H. sapiens (human)
• Insert Size (bp): 965
• GenBank ID: NM_001154
• Entrez Gene: ANXA5 (a.k.a. ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde I (not destroyed)
• 3′ cloning site: Bam HI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry