SupD v2
(Plasmid #198598)

• Purpose: Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.
• Depositing Lab: Jesse Rinehart
• Publication: Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.

Backbone

• Vector backbone: pKD is derived from pKK223-3 (Pharmacia).
• Backbone size w/o insert (bp): 4500
• Total vector size (bp): 4926
• Modifications to backbone: 2 copies of SupD locus
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SupD
• Alt name: Serine supresor tRNA (UAG-Amber)
• Species: Synthetic
• Insert Size (bp): 432

Resource Information

• Supplemental Documents:
  • Rogulina_V4U_1_G30_pLann.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid encodes a serine amber (TAG) suppressor tRNA. Another plasmid encoding a protein of interest with TAG at the proper position is needed for recombinant protein expression. See article and supplementary information for cloning information. This is an improved version of Plasmid 68307: SupD.