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pRS305-mAID-Nb(VHHGFP4)
(Plasmid #198412)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198412 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS305
  • Backbone size w/o insert (bp) 4237
  • Total vector size (bp) 6754
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mAID-Nb (VHHGFP4)
  • Insert Size (bp)
    582
  • Promoter ADH1 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site SacII (unknown if destroyed)
  • 5′ sequencing primer GGTTCCACTAGTATGAgGGAGAgGAGTGCTTGTCC
  • 3′ sequencing primer GGTTCCCCGCGGTTAgctgctaacggtaacct
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS305-mAID-Nb(VHHGFP4) was a gift from Takumi Kamura (Addgene plasmid # 198412 ; http://n2t.net/addgene:198412 ; RRID:Addgene_198412)

  • For your References section:

    Development of AlissAID system targeting GFP or mCherry fusion protein. Ogawa Y, Nishimura K, Obara K, Kamura T. PLoS Genet. 2023 Jun 14;19(6):e1010731. doi: 10.1371/journal.pgen.1010731. eCollection 2023 Jun. 10.1371/journal.pgen.1010731 PubMed 37315088
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