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Addgene

pLenti6.3 GCN1 R2312A-3xFlag_IRES-iRFP
(Plasmid #198389)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198389 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6.3 (SV40-bsd removed)
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    iRFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCN1
  • Species
    H. sapiens (human)
  • Mutation
    R2312A mutation in RWDBD (RWD binding domain)
  • Entrez Gene
    GCN1 (a.k.a. GCN1L, GCN1L1, PRIC295)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFlag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6.3 GCN1 R2312A-3xFlag_IRES-iRFP was a gift from Jack Taunton (Addgene plasmid # 198389 ; http://n2t.net/addgene:198389 ; RRID:Addgene_198389)

  • For your References section:

    An E3 ligase network engages GCN1 to promote the degradation of translation factors on stalled ribosomes. Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J. Cell. 2023 Jan 19;186(2):346-362.e17. doi: 10.1016/j.cell.2022.12.025. Epub 2023 Jan 12. 10.1016/j.cell.2022.12.025 PubMed 36638793
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